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AMT RPT Practice Test: 30 Free Phlebotomy Questions

30 AMT RPT-style questions with answers and explanations, weighted to the American Medical Technologists's Registered Phlebotomy Technician exam. The real exam has 200 scored questions (amt exams may also include unscored pretest items) in 2.5 hours, and you need a scaled score of 70 to pass. No account needed: tap an answer to see why, and get your score and weakest area at the end.

Written by Benjamin Tibbs, a phlebotomy studentChecked against CLSI, OSHA, CDC and The Joint CommissionUpdated October 2026

  1. Question 1 · Obtaining Blood Samples

    During a venipuncture with an evacuated tube system, a large hematoma rapidly forms at the site after two tubes are filled. One more tube is needed. What should the phlebotomist do?

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    Answer: B. Release the tourniquet, remove the needle, and apply firm pressure

    A rapidly growing hematoma means blood is leaking into the tissue, so the draw must stop. Release the tourniquet, withdraw the needle and activate the safety device, and hold firm direct pressure until bleeding stops. Collect the remaining tube from another site; a wrapped cold pack may be applied afterward, but never ice directly on the skin.

    Review: Venipuncture Steps

  2. Question 2 · Obtaining Blood Samples

    A patient with diabetes and peripheral neuropathy has accessible veins only in the feet. What must the phlebotomist confirm before proceeding?

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    Answer: D. That the physician has given permission for a foot draw

    Most facilities require physician permission before drawing from the legs or feet, and standard texts advise against it in diabetic patients without that permission. Poor circulation and neuropathy raise the risk of infection, slow healing, and unnoticed injury, and lower-extremity punctures carry a higher risk of clots. The physician weighs these risks against the need for the specimen.

    Review: Anatomy & Circulatory System

  3. Question 3 · Obtaining Blood Samples

    During site selection, a phlebotomist feels a vein that is hard and cord-like and does not rebound when pressed. What does this most likely indicate?

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    Answer: A. A sclerosed or thrombosed vein that should not be used

    A hard, cord-like vein that does not rebound is sclerosed (scarred, often from repeated punctures or IV use) or thrombosed (clotted). Blood flow through it is poor or absent, so a puncture is likely to fail and may be painful. Healthy veins feel soft and bouncy and refill after gentle pressure.

    Review: Difficult Venipuncture

  4. Question 4 · Obtaining Blood Samples

    Partway through a draw, the patient begins having rhythmic jerking movements of the arm being used, consistent with a seizure. What is the priority action?

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    Answer: C. Remove the needle, call for help, and do not restrain the patient

    The jerking movements can drive the needle through the vein or cause a needlestick, so remove the needle at once and activate the safety device. Call for help, protect the patient from falls and injury, and do not restrain the limbs, which can cause injury. Note the time the seizure started, and do not resume the draw until the patient has been evaluated.

  5. Question 5 · Obtaining Blood Samples

    A phlebotomist draws a lavender-top (EDTA) tube for a CBC and then a green-top (lithium heparin) tube for a chemistry panel that includes calcium and potassium. Because the order of draw was reversed, which results are most likely affected?

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    Answer: B. Calcium and potassium in the green-top tube, because of EDTA carryover

    Lavender is drawn after green so that EDTA cannot carry over on the needle into other tubes. Potassium EDTA binds calcium and contains potassium, so carryover can falsely lower calcium and falsely raise potassium in the heparin tube. The EDTA tube itself, drawn first, is not affected.

    Review: Order of Draw

  6. Question 6 · Specimen Collection & Processing

    A patient collecting a guaiac-based fecal occult blood test ate a large steak and took vitamin C supplements the night before. How do these affect the test?

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    Answer: A. Red meat can cause a false positive; vitamin C can cause a false negative

    Guaiac tests detect the peroxidase activity of hemoglobin. Red meat contains animal hemoglobin and can cause a false positive, while vitamin C blocks the reaction and can cause a false negative. Patients are usually told to avoid red meat, and vitamin C above 250 mg a day, for 3 days before testing; immunochemical tests (FIT) detect only human hemoglobin and do not have these diet limits.

  7. Question 7 · Specimen Collection & Processing

    After centrifugation, the serum for a comprehensive metabolic panel is milky white. The patient ate a large meal an hour before the draw. How does this affect testing?

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    Answer: D. The cloudiness can interfere with photometric tests for several analytes

    Lipemia, cloudy or milky serum caused by excess fats in the blood, often follows a recent meal. The cloudiness scatters light in photometric methods and can falsely raise or lower several results. The lab decides whether to report, treat (for example, by high-speed centrifugation), or reject the specimen based on how severe it is.

    Review: Preanalytical Errors

  8. Question 8 · Time Management & Organization

    At 7:50 AM a phlebotomist has four pending draws: a STAT troponin on a cardiac patient, a routine CBC, an 8:00 AM timed cortisol, and a fasting glucose on a patient whose breakfast arrives at 8:15. What is the best order?

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    Answer: B. STAT troponin → cortisol → fasting glucose → routine CBC

    A STAT order comes first because its result is needed for urgent care, here a possible heart attack. The 8:00 cortisol is next because its timing is part of the test, and the fasting glucose must be drawn before the 8:15 breakfast tray arrives. The routine CBC has no time limit, so it goes last.

  9. Question 9 · Time Management & Organization

    A gentamicin peak is ordered 30 minutes after the IV infusion ends, and a trough 30 minutes before the next dose. The infusion ends at 10:00 AM and the next dose is due at 6:00 PM. When should each be drawn?

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    Answer: D. Peak at 10:30 AM, trough at 5:30 PM

    The peak is drawn 30 minutes after the infusion ends (10:00 + 30 minutes = 10:30 AM). The trough is drawn 30 minutes before the next dose (6:00 PM − 30 minutes = 5:30 PM), when the drug level is lowest. A draw at the wrong time gives a misleading level for dose adjustment.

  10. Question 10 · Professional Communications

    A patient speaks only Mandarin. No in-person interpreter is available, but the hospital has a phone interpreter service, and the patient's English-speaking adult child offers to translate. What is the MOST appropriate action?

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    Answer: C. Use the hospital's phone interpreter service

    Facilities that receive federal funds must provide qualified language assistance to patients with limited English proficiency (Title VI of the Civil Rights Act; Section 1557 of the Affordable Care Act). A trained medical interpreter, by phone or video, conveys identification questions, consent, and instructions accurately, while family members may leave out or change information. Waiting for an in-person interpreter delays care when a qualified remote one is available.

    Review: Patient Identification

  11. Question 11 · Clerical Skills & Duties

    While reconciling the morning draw list, a phlebotomist discovers that a tube labeled for patient Johnson (Room 412) was actually drawn from patient Jensen (Room 414). What must the phlebotomist do?

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    Answer: D. Do not use the specimen; report the error and recollect correctly labeled specimens from the patients involved

    A mislabeled specimen has lost its proof of identity and cannot be fixed by relabeling, crossing out, or attaching a note. It must not be used; the error is reported through the facility's incident system, and new specimens are collected with labels applied at the bedside. Johnson's ordered tests still need to be drawn, and any of Jensen's specimens from the same round must be checked and recollected if affected.

    Review: Patient Identification

  12. Question 12 · Safety Standards & Procedures

    A phlebotomist is about to enter a room posted for Droplet Precautions. Beyond standard precautions, what PPE is required?

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    Answer: B. A surgical mask

    Droplet precautions apply to infections spread by large respiratory droplets over short distances, such as influenza, pertussis, and meningococcal disease. CDC guidelines add a surgical (procedure) mask, put on before entering the room, to standard precautions. N95 respirators are for airborne precautions, such as tuberculosis, measles, and chickenpox.

    Review: Infection Control

  13. Question 13 · Safety Standards & Procedures

    During a C. difficile outbreak on the unit, a phlebotomist finishes a draw on an infected patient and removes their gloves before leaving the room. Which hand hygiene method is preferred?

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    Answer: C. Soap and water, because alcohol does not kill C. difficile spores

    Alcohol does not kill C. difficile spores, and washing with soap and water removes them from the hands more effectively. During outbreaks, IDSA/SHEA guidance prefers soap and water after caring for patients with C. difficile, and the CDC encourages it; in routine settings either method is acceptable unless hands are visibly soiled. Gloves and a gown under contact precautions are the main protection, and wearing gloves never replaces hand hygiene.

    Review: Infection Control

  14. Question 14 · Legal, Ethical & Professional

    A competent adult patient firmly refuses a physician-ordered blood draw after the phlebotomist explains why the test matters. What is the legally correct action?

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    Answer: A. Respect the refusal, document it, and notify the nurse or provider

    Competent adults have the right to refuse any procedure, even one a physician ordered. Drawing blood against a patient's wishes can be treated as assault and battery. The phlebotomist documents the refusal according to facility policy and notifies the nurse or ordering provider; threats and force are never acceptable.

  15. Question 15 · Terminology, Anatomy & Physiology

    Which sequence correctly describes the path of blood through the heart and lungs?

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    Answer: C. Right atrium → right ventricle → pulmonary artery → lungs → pulmonary veins → left atrium → left ventricle → aorta

    Oxygen-poor blood enters the right atrium from the venae cavae, passes through the tricuspid valve into the right ventricle, and is pumped through the pulmonary artery to the lungs. Oxygen-rich blood returns through the pulmonary veins to the left atrium, passes through the mitral valve into the left ventricle, and is pumped out through the aorta to the body.

    Review: Anatomy & Circulatory System

  16. Question 16 · Obtaining Blood Samples

    An outpatient is called in for a blood draw. Which is the correct way to confirm her identity?

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    Answer: A. Have her state her name and birth date, then check the requisition

    The patient states her own identifiers in answer to open-ended questions, and they are compared with the requisition (The Joint Commission requires at least two identifiers). A yes-or-no question invites a wrong 'yes' from a patient who is anxious, hard of hearing or confused.

    Review: Venipuncture Steps

    Why the other choices are wrong

    • B. A patient may answer 'yes' to a name that is not hers because she misheard or is nervous. Have her state it.
    • C. Reading the identifiers aloud lets the patient agree without really checking. She should state them herself.
    • D. An insurance card and an appointment list do not confirm who is in the chair. The patient states her identifiers and they are matched to the requisition.
  17. Question 17 · Obtaining Blood Samples

    During a routine venipuncture, the blood is bright red and spurts into the tube in pulses. What should the phlebotomist do?

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    Answer: B. Remove the needle, hold firm pressure 5 minutes or more, and report it

    Bright red blood that pulses into the tube signals an accidental arterial puncture. Remove the needle and apply firm pressure for at least 5 minutes (longer if the patient takes anticoagulants) to prevent a hematoma, check the site, and tell the nurse or supervisor. If the specimen is kept, the laboratory must be told it may be arterial.

    Review: Venipuncture Steps

    Why the other choices are wrong

    • A. Arterial blood is under pressure. Brief pressure after an arterial puncture leads to a hematoma.
    • C. Loosening the tourniquet does not stop arterial flow, and leaving the needle in an artery prolongs the injury.
    • D. Arterial and venous values differ for some tests, such as blood gases, so the specimen must not be passed off as venous.
  18. Question 18 · Obtaining Blood Samples

    Which finger is preferred for an adult fingerstick?

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    Answer: C. The middle or ring finger

    CLSI GP42-Ed7 recommends the palm side of the fingertip of the middle or ring finger, slightly off center and across the fingerprint lines. The thumb has a pulse and thick skin, the index finger is usually more callused and sensitive, and the little finger has so little tissue that the bone is close to the surface.

    Review: Venipuncture Steps

    Why the other choices are wrong

    • A. The thumb has a pulse, with an artery close by, and thick, callused skin.
    • B. The index finger is usually more callused and more sensitive to pain.
    • D. The little finger has so little tissue that the bone lies close to the skin.
  19. Question 19 · Obtaining Blood Samples

    Five minutes after a venipuncture, the site is still bleeding despite pressure. What should the phlebotomist do?

    Show answer

    Answer: D. Keep up the pressure and alert the nurse if it does not stop

    The phlebotomist stays with the patient and keeps pressure on the site until bleeding stops. Bleeding that continues beyond about 5 minutes is reported to the nurse or provider, since it can signal anticoagulant therapy or a clotting problem. A patient is never left with a site that is still bleeding.

    Review: Venipuncture Steps

    Why the other choices are wrong

    • A. A bandage over a site that is still bleeding hides the problem and can lead to a hematoma.
    • B. The phlebotomist is responsible for the site until bleeding stops. Leaving the patient alone with it is unsafe.
    • C. Leaving a patient whose site is still bleeding is the error. The nurse or provider must be told.
  20. Question 20 · Obtaining Blood Samples

    A new phlebotomist shakes each filled tube hard 'to make sure the additive mixes.' What is wrong with this?

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    Answer: A. Shaking can hemolyze it; tubes are inverted gently

    Shaking ruptures red cells (hemolysis), which falsely raises potassium and other analytes and can make the specimen unacceptable. Additive tubes are mixed by gentle, complete inversions right after filling, the number set by the manufacturer: for BD tubes, 3 to 4 for citrate, 5 for serum tubes, and 8 to 10 for heparin, EDTA and fluoride.

    Review: Venipuncture Steps

    Why the other choices are wrong

    • B. No tube is shaken. EDTA tubes are inverted gently 8 to 10 times.
    • C. Plain tubes with no additive need no mixing at all. Additive tubes are inverted gently, never shaken.
    • D. Hemolysis happens inside the tube whether or not the stopper stays on.
  21. Question 21 · Specimen Collection & Processing

    A serum specimen will be shipped by ground courier to a reference laboratory as UN3373, 'Biological Substance, Category B.' How must it be packaged?

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    Answer: B. Leakproof tube, sealed secondary with absorbent, rigid outer box

    Category B infectious substances (UN3373) are shipped in triple packaging under 49 CFR 173.199 and IATA Packing Instruction 650: a leakproof primary receptacle, a leakproof secondary packaging with enough absorbent to soak up the entire contents, and a rigid outer packaging marked UN3373 with the proper shipping name.

    Review: Specimen Handling

    Why the other choices are wrong

    • A. A padded envelope is not a rigid outer packaging, and one bag is not the required three layers.
    • C. A cooler can keep the temperature, but the specimen still needs a leakproof secondary with absorbent and the UN3373 marking.
    • D. The box must hold the primary and secondary layers with absorbent, and it is marked UN3373, not only with a biohazard symbol.
  22. Question 22 · Specimen Collection & Processing

    A patient is starting a 24-hour urine collection at 7 AM. Which instruction is correct?

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    Answer: C. Empty the bladder at 7 AM and discard it, then save all urine until 7 AM tomorrow

    A timed 24-hour collection starts with an empty bladder: the 7 AM urine is discarded and the start time recorded, then all urine for the next 24 hours is saved, ending with a final void at 7 AM the next day, which is included. The container is refrigerated or kept as the laboratory instructs.

    Review: Specimen Handling

    Why the other choices are wrong

    • A. The 7 AM urine was made before the collection period began. Including it adds urine from outside the 24 hours.
    • B. A 24-hour test needs every void in the period, not just the morning ones.
    • D. Timing and completeness are the test. Missed or extra urine makes the result wrong.
  23. Question 23 · Time Management & Organization

    A phlebotomist arrives for an 11:00 AM timed cortisol draw, but the patient has been taken to radiology. What is the best action?

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    Answer: D. Tell the nurse and document why the timed draw was missed

    A timed draw that cannot be done on time is a problem to refer, not to solve alone. The nurse is told, the delay is documented, and facility policy decides whether to collect late, with the actual time recorded, or to reschedule. A label always shows the actual collection time; cortisol varies through the day, so the time matters.

    Why the other choices are wrong

    • A. Labeling with a time the specimen was not drawn falsifies the record, and cortisol results depend on the time of day.
    • B. Cancelling an order is not the phlebotomist's decision. The nurse or provider decides.
    • C. Waiting indefinitely delays every other draw on the list. Refer the problem and move on.
  24. Question 24 · Professional Communications

    Before a draw, a nervous patient asks, 'Is this going to hurt?' Which reply is the most professional?

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    Answer: A. A quick pinch, and I'll warn you right before

    Patients are owed honest, calm information: a brief sting is expected, and a warning just before the needle goes in reduces startled movement. Promising no pain costs trust when it does hurt, exaggerating it raises anxiety, and brushing the question off is dismissive.

    Why the other choices are wrong

    • B. Most patients feel a brief sting. Promising no pain costs the patient's trust as soon as the needle goes in.
    • C. Exaggerating the pain makes a nervous patient more anxious and more likely to move or faint.
    • D. Brushing off the question is dismissive and does nothing to calm the patient.
  25. Question 25 · Clerical Skills & Duties

    An outpatient requisition lists the patient's name, date of birth and tests, but no ordering provider. What should the phlebotomist do?

    Show answer

    Answer: B. Get the order completed by the provider's office first

    Under CLIA (42 CFR 493.1241), a laboratory tests only on the request of an authorized person, and the requisition must identify that person so results and critical values can be reported. A missing provider is resolved with the ordering office before the draw; the phlebotomist does not guess or fill it in.

    Review: Patient Identification

    Why the other choices are wrong

    • A. Without an authorized orderer the laboratory may not test the specimen, and critical results would have no one to go to.
    • C. Guessing the orderer creates a false record, and results could go to a provider who never ordered them.
    • D. The patient's recollection is not an authorized order. The ordering office must confirm it.
  26. Question 26 · Safety Standards & Procedures

    Under Standard Precautions, which of these is NOT treated as potentially infectious?

    Show answer

    Answer: C. Sweat

    CDC's Standard Precautions rest on the principle that blood, all body fluids, secretions and excretions except sweat, nonintact skin and mucous membranes may carry transmissible infectious agents. They apply to every patient, whatever the diagnosis.

    Review: Infection Control

    Why the other choices are wrong

    • A. Blood is the central concern of both Standard Precautions and OSHA's Bloodborne Pathogens Standard.
    • B. Wound drainage is a body fluid and is treated as potentially infectious.
    • D. Saliva is a secretion, and Standard Precautions cover all secretions.
  27. Question 27 · Safety Standards & Procedures

    On a chemical storage cabinet, an NFPA 704 hazard diamond shows a 3 in the blue section. What does this mean?

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    Answer: D. A serious health hazard

    In the NFPA 704 diamond, blue is health, red is flammability, yellow is instability (reactivity) and white is for special hazards. Ratings run from 0 (minimal) to 4 (severe), so a 3 in blue means a serious health hazard.

    Review: Infection Control

    Why the other choices are wrong

    • A. Fire (flammability) is the red section.
    • B. Instability or reactivity is the yellow section.
    • C. Special hazards, such as water reactivity or oxidizers, go in the white section and are shown as symbols, not numbers.
  28. Question 28 · Safety Standards & Procedures

    How often does OSHA's Bloodborne Pathogens Standard require bloodborne pathogens training for phlebotomists?

    Show answer

    Answer: A. When first assigned, then at least yearly

    OSHA 29 CFR 1910.1030(g)(2) requires training when an employee is first assigned to tasks with occupational exposure and at least annually after that, at no cost to the employee and during working hours. Training records are kept for 3 years.

    Review: Infection Control

    Why the other choices are wrong

    • B. One-time training is not enough. It must be repeated at least every year.
    • C. Certification renewal is set by the certifying body, not OSHA. OSHA requires training at least annually.
    • D. Training must come before the exposure risk begins, not after an exposure.
  29. Question 29 · Legal, Ethical & Professional

    A hospital phlebotomist injures a patient through careless technique while on duty, and the patient sues the hospital. Which legal doctrine makes the employer responsible for the employee's acts?

    Show answer

    Answer: B. Respondeat superior

    Respondeat superior ('let the master answer') makes an employer legally responsible for the negligent acts of employees done within the scope of their job. It does not remove the employee's own duty to meet the standard of care.

    Why the other choices are wrong

    • A. Res ipsa loquitur ('the thing speaks for itself') lets negligence be inferred from the injury itself. It does not assign liability to an employer.
    • C. Informed consent concerns the patient's agreement to a procedure, not the employer's liability.
    • D. The statute of limitations sets how long a patient has to file a lawsuit.
  30. Question 30 · Legal, Ethical & Professional

    A 15-year-old who is not emancipated comes in alone for a routine cholesterol screening ordered by her pediatrician. What should the phlebotomist do about consent?

    Show answer

    Answer: A. Get a parent's or guardian's consent first

    As a rule, a minor cannot give legal consent for routine medical procedures: a parent or legal guardian consents, unless the minor is emancipated or a state law makes an exception (some states let minors consent to specific services, such as testing for sexually transmitted infections). A routine cholesterol screening falls under the general rule, so follow facility policy and obtain a parent's or guardian's consent.

    Why the other choices are wrong

    • B. Implied consent from a minor is not valid consent for a routine procedure.
    • C. A non-emancipated minor's signature is not legal consent for a routine procedure.
    • D. A provider's order authorizes the test; it does not replace the consent of the patient or, for a minor, the guardian.

More free practice tests

Every set has different questions, each with an explanation and your score by domain. No account needed.

The three phlebotomy certification exams

Question counts, time limits and passing scores from each certifying body's own documents, checked October 6, 2026.

ExamQuestionsTimePassing scorePractice
NHA CPTNational Healthcareer Association120 questions: 100 scored and 20 unscored pretest items2 hours390 (scaled score from 200 to 500)NHA CPT practice exam
ASCP PBTAmerican Society for Clinical Pathology Board of Certification80 multiple-choice questions2 hours400 (scaled score)ASCP PBT practice exam
AMT RPTAmerican Medical Technologists200 scored questions (AMT exams may also include unscored pretest items)2.5 hours70 (scaled score from 0 to 100)This page

More detail: how each exam is scored · retake rules · NHA vs ASCP vs AMT · all exam facts with sources · general phlebotomy practice test

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